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KMID : 1094020200370010023
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Journal of Veterinary Clinics
2020 Volume.37 No. 1 p.23 ~ p.27
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Ultra Fast Real-Time PCR for Detection of Babesia gibsoni as Point of Care Test
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Yang Yong-Sung
Mun Myung-Jun
Yun Young-Min
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Abstract
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Between May and November 2018, babesiosis was examined in 162 bloods samples obtained to an animal hospital in Jeju island for anemia and medical examination. Sixty-two of 162 (38.3%) were positive by PCR. The ultra fast real-time PCR test with blood directly analyzed without DNA extraction showed the same results. Accurate diagnosis, treatment and prognosis of babesiosis should be combined with clinical symptoms, blood tests, the babesia antibody test, and the PCR antigen test. Ultra fast real-time PCR, with these tests, is expected to be a point-of-care testing (POCT) for easy, fast and accurate diagnosis of babesiosis in the veterinary clinic.
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KEYWORD
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babesia gibsoni, ultra fast real-time PCR, POCT, dog
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